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Directed Evolution Library Creation: Methods and Protocols Frances H. Arnold Editor
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Biological systems are very special substrates for engineering—uniquely the products of evolution, they are easily redesigned by similar approaches. A simple algorithm of iterative … Plus…

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Directed Evolution Library Creation: Methods And Protocols (Methods In Molecular Biology, Volume 231) - nouveau livre

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Directed Evolution Library Creation: Methods and Protocols Frances H. Arnold Editor

A comprehensive compendium of cutting-edge protocols for the generation of molecular diversity. Described in step-by-step detail to ensure experimental success, these protocols include readily reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologus recombination, and for constructing in vivo libraries in bacteria and yeast. In addition to the various protocols for creating libraries, this volume also describes ways to analyze libraries, particularly those made by recombination. An accompanying volume, Directed Enzyme Evolution: Screening and Selection Methods (ISBN: 1-58829-286-X), is devoted entirely to selection and screening methods that can be applied to the directed evolution of enzymes. Copy for Both Volumes Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods constitute an extraordinary collection of all the key methods used today for directed evolution research. Described in step-by-step detail to ensure robust experimental results, these methods will enable both newcomers and more experienced investigators to design and implement directed evolution strategies for the engineering of novel proteins. The first volume describes methods for the creation of mutated DNA molecules, or DNA libraries, encoding variants of desired proteins. The second volume describes methods for screening DNA libraries to isolate mutant proteins that exhibit a specified function.

Informations détaillées sur le livre - Directed Evolution Library Creation: Methods and Protocols Frances H. Arnold Editor


EAN (ISBN-13): 9781588292858
ISBN (ISBN-10): 1588292851
Version reliée
Livre de poche
Date de parution: 2003
Editeur: Springer-Verlag New York, LLC Core >2 >T
236 Pages
Poids: 0,538 kg
Langue: eng/Englisch

Livre dans la base de données depuis 2007-04-17T07:40:48+02:00 (Paris)
Page de détail modifiée en dernier sur 2024-03-03T18:36:58+01:00 (Paris)
ISBN/EAN: 1588292851

ISBN - Autres types d'écriture:
1-58829-285-1, 978-1-58829-285-8
Autres types d'écriture et termes associés:
Auteur du livre: hamilton george, george georgiou, leandro pena, arnold, georgi
Titre du livre: molecular evolution, dmc creation, library, methods


Données de l'éditeur

Auteur: Frances H. Arnold; George Georgiou
Titre: Methods in Molecular Biology; Directed Evolution Library Creation - Methods and Protocols
Editeur: Humana; Humana Press
226 Pages
Date de parution: 2003-04-25
Totowa; NJ; US
Poids: 0,542 kg
Langue: Anglais
106,99 € (DE)
109,99 € (AT)
118,00 CHF (CH)
POD
X, 226 p.

BB; Cell Biology; Hardcover, Softcover / Biologie/Mikrobiologie; Zellbiologie (Zytologie); Verstehen; Cell Biology; BC; EA

Part I. Mutagenesis and Recombination Methods Generating Mutant Libraries Using Error-Prone PCR Patrick C. Cirino, Kimberly M. Mayer, and Daisuke Umeno Preparing Libraries in Escherichia coli Alexander V. Tobias Preparing Libraries in Saccharomyces cerevisiae Thomas Bulter and Miguel Alcalde Creating Random Mutagenesis Libraries by Megaprimer PCR of Whole Plasmid (MEGAWHOP) Kentaro Miyazaki Construction of Designed Protein Libraries Using Gene Assembly Mutagenesis Paul H. Bessette, Marco A. Mena, Annalee W. Nguyen, and Patrick S. Daugherty Production of Randomly Mutated Plasmid Libraries Using Mutator Strains Annalee W. Nguyen and Patrick S. Daugherty Evolution of Microorganisms Using Mutator Plasmids Olga Selifonova and Volker Schellenberger Random Insertion and Deletion Mutagenesis Hiroshi Murakami, Takahiro Hohsaka, and Masahiko Sisido Random Oligonucleotide Mutagenesis Jessica L. Sneeden and Lawrence A. Loeb Saturation Mutagenesis Radu Georgescu, Geethani Bandara, and Lianhong Sun DNA Shuffling John M. Joern Family Shuffling with Single-Stranded DNA Wenjuan Zha, Tongbo Zhu, and Huimin Zhao In Vitro DNA Recombination by Random Priming Olga Esteban, Ryan D. Woodyer, and Huimin Zhao Staggered Extension Process (StEP) In Vitro Recombination Anna Marie Aguinaldo and Frances H. Arnold RACHITT: Gene Family Shuffling by Random Chimeragenesis on Transient Templates Wayne M. Coco The Creation of ITCHY Hybrid Protein Libraries Marc Ostermeier and Stefan Lutz Preparation of SCRATCHY Hybrid Protein Libraries: Size- and In-Frame Selection of Nucleic Acid Sequences Stefan Lutz and Marc Ostermeier Sequence Homology-Independent Protein Recombination (SHIPREC) Andrew K. Udit, Jonathan J. Silberg,and Volker Sieber Producing Chimeric Genes by CLERY: In Vitro and In Vivo Recombination Valérie Abécassis, Denis Pompon, and Gilles Truan Part II. Analysis of Library Diversity Analysis of Shuffled Libraries by Oligonucleotide Probe Hybridization Peter Meinhold, John M. Joern, and Jonathan J. Silberg Sequence Mapping of Combinatorial Libraries on Macro- or Microarrays: Experimental Design of DNA Arrays Valérie Abécassis, Gilles Truan, Loïc Jaffrelo, and Denis Pompon Sequence Mapping of Combinatorial Libraries on Macro- and Microarrays: Bioinformatic Treatment of Data Denis Pompon, Gilles Truan, and Valérie Abécassis Index

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