Auditory and Vestibular Research: Methods and Protocols Bernd Sokolowski Editor - nouveau livre

Livre dans la base de données depuis 2018-08-01T10:39:42+02:00 (Paris)
Page de détail modifiée en dernier sur 2024-03-26T20:36:19+01:00 (Paris)
ISBN/EAN: 1493980955

ISBN - Autres types d'écriture:
1-4939-8095-5, 978-1-4939-8095-6
Autres types d'écriture et termes associés:
Auteur du livre: sokolowski, sokol, sokolow
Titre du livre: sokolowski

Données de l'éditeur

Auteur: Bernd Sokolowski
Titre: Methods in Molecular Biology; Auditory and Vestibular Research - Methods and Protocols
Editeur: Humana; Springer US
547 Pages
Date de parution: 2018-05-26
New York; NY; US
Imprimé / Fabriqué en
Langue: Anglais
117,69 € (DE)
120,99 € (AT)
130,00 CHF (CH)
POD
XVI, 547 p. 120 illus., 89 illus. in color.

BC; Hardcover, Softcover / Medizin/Nichtklinische Fächer; Medizinische Forschung; Verstehen; tissue engineering; nanotechnology; high-throughput screening; physiology; optical coherence tomography; patch clamping; Biomedical Research; Medical Genetics; Genetik, Medizin; BB

series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

aims to ensure successful results in the further study of this vital field.

 Helios® Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium: Recent Updates.- Tol2-Mediated Delivery of miRNAs to the Chicken Otocyst using Plasmid Electroporation.-  A Rapid, Cost-Effective Method of Recombinant Adeno-Associated Virus Preparation Enables Efficient Gene Transfer to the Developing Mouse Inner Ear.-  Generation of Noninvasive, Quantifiable, Orthotopic Animal Models for NF2-Associated Schwannoma and Meningioma.- RNA Extraction from Xenopus Auditory and Vestibular Organs for Molecular Cloning and Expression Profiling with RNA-Seq and Microarrays .- Yeast Two-Hybrid Screening to Test for Protein-Protein Interactions in the Auditory System.-  Multiplexed Isobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches to Auditory Research.- Protein Quantification of the Developing Cochlea by Mass Spectrometry.-  Ultrastructural Identification and Colocalization of Interacting Proteins in the Murine Cochlea by Post-Embedding Immunogold Transmission Electron Microscopy.- Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Inner-Ear Proteins.- The Single-Molecule Approach to Membrane Protein Stoichiometry.- Visualization of Live Cochlear Stereocilia at a Nanoscale Resolution Using Hopping Probe Ion Conductance Microscopy.- Design and Construction of a Cost-Effective Spinning Disk System for Live Imaging of Inner Ear Tissue.- Neuroanatomical Tracing Techniques: History, State of the Art, and Future Developments.-  Detection of Excitation and Inhibition in the Auditory System Using Fluorescence Immunohistochemistry and High Resolution Fluorescence Microscopy.-  Rapid and Semi-Automated Extraction of Neuronal Cell Bodies and Nuclei from Electron Microscopy Image Stacks.-Organotypic Culture of the Mouse Cochlea from Embryonic Day 12 to the Neonate.- Quantifying Spiral Ganglion Neurite and Schwann Behavior on Micropatterned Polymer Substrates.- The Use of Human Wharton’s Jelly Cells for Cochlear Tissue Engineering.- Nanotechnology in Auditory Research: Membrane Electromechanics in Hearing.- Overview of Nanoparticle Based Delivery for Treatment of Inner Ear Disorders.-  Development of Cell-based High-Throughput Chemical Screens for Protection Against Cisplatin-induced Ototoxicity.-Profiling Specific Inner Ear Cell Types Using Cell Sorting Techniques.- Optical Coherence Tomography to Measure Sound-Induced Motions Within the Mouse Organ of Corti In Vivo.- Method for Dissecting the Auditory Epithelium (Basilar Papilla) in Developing Chick Embryos.-  Whole Cell Patch Clamp Recording of Mouse and Rat Inner Hair Cells in the Intact Organ of Corti.-  Glass Probe Stimulation of Hair Cell Stereocilia.- A Walkthrough of Nonlinear Capacitance Measurement of Outer Hair Cells.- In Vitro Functional Assessment of Adult Spiral Ganglion Neurons (SGNs).- Mapping Auditory Circuits with Photostimulation of Caged Glutamate.

series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

aims to ensure successful results in the further study of this vital field.