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This volume provides a thorough overview of the Wilms’ Tumour Gene (WT1). The book begins with three review chapters that cover the involvement of WT1 in pediatric cancer, kidney di… Plus…

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The Wilms' Tumor (WT1) Gene - edition reliée, livre de poche

2016, ISBN: 9781493940219

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The Wilms' Tumor (WT1) Gene

This volume provides a thorough overview of the Wilms’ Tumour Gene (WT1). The book begins with three review chapters that cover the involvement of WT1 in pediatric cancer, kidney disease, and tissue development and homeostasis. The next few chapters discuss cell marking and lineage tracing, epicardial cell methodology, colony forming assays for bone marrow stem cells, angiogenesis assays and zebrafish tools.  The next group of chapters explores the latest tools in genomics, molecular biology, and biochemistry. They discuss dissecting transcription factor function in cell free systems, ChiP seq, proteomics, RNA interactome, and multiphoton imaging of lipids, measuring the binding constants of protein-nucleic acid interactions, and bioinformatics approaches for analyzing Next Generation Sequence data. The final chapter discusses protocols for clinical trials for immune therapy using anti-WT1 peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, The Wilms’ Tumour (WT1) Gene: Methods and Protocols is a valuable resource for anyone who is interested in the diverse methodologies used in WT1 research.

 

Informations détaillées sur le livre - The Wilms' Tumor (WT1) Gene


EAN (ISBN-13): 9781493940219
ISBN (ISBN-10): 149394021X
Version reliée
Date de parution: 2016
Editeur: Humana Press Inc.

Livre dans la base de données depuis 2016-09-18T21:08:34+02:00 (Paris)
Page de détail modifiée en dernier sur 2023-01-16T11:11:15+01:00 (Paris)
ISBN/EAN: 9781493940219

ISBN - Autres types d'écriture:
1-4939-4021-X, 978-1-4939-4021-9
Autres types d'écriture et termes associés:
Auteur du livre: nicholas
Titre du livre: wilms tumor, molecular biology the gene, ships wanderings schwarze negras negres


Données de l'éditeur

Auteur: Nicholas Hastie
Titre: Methods in Molecular Biology; The Wilms' Tumor (WT1) Gene - Methods and Protocols
Editeur: Humana; Springer US
235 Pages
Date de parution: 2016-07-20
New York; NY; US
Imprimé / Fabriqué en
Langue: Anglais
106,99 € (DE)
109,99 € (AT)
118,00 CHF (CH)
POD
X, 235 p. 42 illus., 34 illus. in color.

BB; Hardcover, Softcover / Medizin/Nichtklinische Fächer; Onkologie; Verstehen; multifunctional protein; mesenchymal-epithelial switches; zinc finger protein; cell making; lineage tracing; zebrafish; protein-nucleic acid interaction; ChIP-Seq; WT1-interacting RNA; cancer immune therapy; Cancer Biology; Medical Genetics; Genetik, Medizin; EA; BC

is a valuable resource for anyone who is interested in the diverse methodologies used in WT1 research.

Methods in Molecular Biology The Wilms’ Tumour (WT1) Gene: Methods and Protocols  

Assays for Assessing the Role of the Wilms’ Tumor Suppressor 1 (WT1) in Angiogenesis.- Multiphoton Microscopy for Visualizing Lipids in Tissue.- Function and Regulation of the Wilm’s Tumor Suppressor 1 (WT1) Gene in Fish.- Immunofluorescence Staining of WT1 on Sections of Zebrafish Embryos and Larvae.- Fluorescence Activated Cell Sorting (FACS) Protocol for Podocyte Isolation in Adult Zebrafish.- In Vitro Transcription to Study WT1 Function.- Measuring Equilibrium Binding Constants for the WT1-DNAInteraction Using a Filter Binding Assay.- Identifying Direct Downstream Targets: WT1 ChIP-Seq Analysis.- WT1 Associated Protein-Protein Interaction Networks.- Methods to Identify and Validate WT1-RNA Interaction.- Bioinformatic Analysis of Next Generation Sequencing Data to Identify WT1 Associated Differential Gene and Isoform Expression.- Immunotherapy Targeting WT1: Designing a Protocol for WT1 Peptide-Based Cancer Vaccine.

In Vivo

is a valuable resource for anyone who is interested in the diverse methodologies used in WT1 research.

Methods in Molecular Biology The Wilms’ Tumour (WT1) Gene: Methods and Protocols  
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains keynotes and implementation advice from the experts Includes supplementary material: sn.pub/extras

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