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Transgenic Plants: Methods and Protocols (Methods in Molecular Biology, 847, Band 847)

In 2010 the global area of transgenic crops reached 148 million hectares, an 87-fold increase since 1996, making it the most rapidly adopted technology in the history of modern agriculture. In Transgenic Plants: Methods and Protocols, Second Edition expert researchers in the field provide key techniques to investigate production and analysis of transgenic plants.  Focusing on selection and detection methods, transformation technology, gene targeting, silencing and directed mutation, metabolic engineering and pharming, the book encompasses protocols relating to major crops and model plants being used for genomic analysis. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 

Thorough and intuitive, Transgenic Plants: Methods and Protocols, Second Edition aids scientists in the continuous improvements being made for the production and analysis of transgenic plants.

Informations détaillées sur le livre - Transgenic Plants: Methods and Protocols (Methods in Molecular Biology, 847, Band 847)


EAN (ISBN-13): 9781493960781
ISBN (ISBN-10): 1493960784
Livre de poche
Date de parution: 2016
Editeur: Dunwell, Jim M. Wetten, Andy C, Humana

Livre dans la base de données depuis 2017-09-30T20:16:59+02:00 (Paris)
Page de détail modifiée en dernier sur 2023-09-07T11:10:01+02:00 (Paris)
ISBN/EAN: 9781493960781

ISBN - Autres types d'écriture:
1-4939-6078-4, 978-1-4939-6078-1
Autres types d'écriture et termes associés:
Auteur du livre: wette
Titre du livre: biology plants


Données de l'éditeur

Auteur: Jim M. Dunwell; Andy C Wetten
Titre: Methods in Molecular Biology; Transgenic Plants - Methods and Protocols
Editeur: Humana; Humana Press
497 Pages
Date de parution: 2016-08-23
Totowa; NJ; US
Imprimé / Fabriqué en
Poids: 1,315 kg
Langue: Anglais
176,54 € (DE)
181,49 € (AT)
195,00 CHF (CH)
POD
XV, 497 p. 92 illus., 30 illus. in color.

BC; Plant Sciences; Hardcover, Softcover / Biologie/Botanik; Botanik und Pflanzenwissenschaften; Verstehen; Pflanzen (Botanik); Transgenic Plants; algae; crop species; dicots; modified metabolism; monocots; pharmaceuticals; plant gene; rice; transgenic Arabidopsis; Transgenic organisms; Plant Genetics and Genomics; Transgenics; Plant Science; Plant Genetics; Experimental Organisms; Pflanzenbiologie; Genetik (nicht-medizinisch); Biologie, Biowissenschaften; Wissenschaftliche Ausstattung, Experimente und Techniken; BB

™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 

aids scientists in the continuous improvements being made for the production and analysis of transgenic plants.

Transgenic Plants: Methods and Protocols, Second Edition Methods in Molecular Biology Transgenic Plants: Methods and Protocols, Second Edition

Contents

 

Preface

 

Contributors

 

Part I. Selection AND DETECTION methods

 

1. Employment of Cytokinin Vectors for Marker-Free and Backbone-Free Transformation

 

      Craig M. Richael and Caius M. Rommens

 

 

 

2.         Organophosphorus Hydrolase; a Multifaceted Plant Genetic Marker Which is Selectable, Scorable, and Quantifiable in Whole Seed

 

T. Scott Pinkerton, James R. Wild, and John A. Howard

 

 

 

3.         Use of Northern Blotting for Specific Detection of Small RNA Molecules in Transgenic Plants

Basel Khraiwesh

 

 

 

Part II. Transformation Technology – Lower Plants

 

4.         Genetic Transformation of the Model Green Alga Chlamydomonas reinhardtii

 

Juliane Neupert, Ning Shao, Yinghong Lu, and Ralph Bock

 

 

 

Part III. Transformation Technology – Rice

 

5.     A High Efficiency Agrobacterium-mediated Transformation System of Rice (Oryza sativa L.)

 

Kenjirou Ozawa

 

 

 

6.         Selection of Transgenic Rice Plants Using a Herbicide Tolerant Form of the Acetolactate Synthase Gene

 

Masaki Endo, Tsutomu Shimizu and Seiichi Toki

 

 

 

7.         Visual Selection in Rice—a Strategy for the Efficient Identification of Transgenic Calli Accumulating Transgene Products

 

Hiroaki Saika, Haruko Onodera and Seiichi Toki

 

 

 

8.         Characterization of Rice Genes using Heterologous a Full-length cDNA Expression System

 

Mieko Higuchi, Youichi Kondou, Masaki Mori, Takanari Ichikawa, Minami Matsui

 

 

 

9. Bioactive Bead-mediated Transformation of Plants with Large DNA Fragments

 

Naoki Wada, Joyce A. Cartagena, Naruemon Khemkladngoen, and Kiichi Fukui

 

 

 

Part IV. Transformation Technology – Cereals and Other Monocots

 

 

 

10.       Agrobacterium-mediated Transformation of Sorghum bicolor Using Immature Embryos

 

Songul Gurel, Ekrem Gurel, Tamara I. Miller, and Peggy G. Lemaux

 

 

 

11.       Split-transgene Expression in Wheat

 

Mario Gils, Myroslava Rubtsova, and Katja Kempe

 

 

 

12.       Agrobacterium-mediated Transformation of Brachypodium Distachyon

 

Vera Thole and Philippe Vain

 

 

 

13.       Transformation of Barley (Hordeum vulgare L.) by Agrobacterium tumefaciens Infection of In Vitro Cultured Ovules

 

Inger Bæksted Holme, Henrik Brinch-Pedersen, Mette Lange and Preben Bach Holm

 

 

 

14.       Biolistic Mediated Production of Transgenic Oil Palm

 

Ghulam Kadir Ahmad Parveez and Bohari Bahariah

 

 

 

15.       Transformation of Oil Palm using Agrobacterium tumefaciens

 

Abang Masli Dayang Izawati, Ghulam Kadir Ahmad Parveez, and Mat Yunus Abdul Masani

 

 

 

Part V. Transformation Technology – Dicots

 

16.       Highly Efficient Transformation Protocol for Plum (Prunus domestica L.)

César Petri, Ralph Scorza, and Chinnathambi Srinivasan

 

 

 

17.       Co-transformation of Grapevine Somatic Embryos to Produce Transgenic Plants Free of Marker Genes

 

Manjul Dutt, Zhijian T. Li, Sandanand A. Dhekney and Dennis J. Gray

 

 

 

18.       Initiation and Transformation of Grapevine Embryogenic Cultures

 

Sandanand A. Dhekney, Zhijian T. Li, Manjul Dutt, and Dennis J. Gray

 

 

 

19.       Development of Highly Efficient Genetic Transformation Protocols for Table Grape Sugraone and Crimson Seedless

 

Mercedes Dabauza and Leonardo Velasco

 

 

 

20.       Cotton Pistil Drip Transformation Method

 

Tianzhen Zhang and Tianzhi Chen

 

 

 

21.       Enhanced Agrobacterium-mediated Transformation of Embryogenic Calli of Upland Cotton

 

Tian-zhen Zhang and Shen-jie Wu

 

 

 

22.       Targeted Biolistics for Improved Transformation of Impatiens balsamina

 

Andy Wetten, Jean-Luc Thomas, Alina Wagiran, Tinashe Chiurugwi

 

 

 

23.       A Protocol for Transformation of Torenia

 

Ryutaro Aida

 

 

 

24.       Efficient Modification of Floral Traits by Heavy-Ion Beam Irradiation on Transgenic Torenia

 

Norihiro Ohtsubo, Katsutomo Sasaki, Ryutaro Aida, Hiromichi Ryuto, Hiroyuki Ichida, Yoriko Hayashi, and Tomoko Abe

 

 

 

Part VI. Gene Targeting, Silencing and Directed Mutation

 

25.      

 

Isam Fattash, Basel Khraiwesh , M. Asif Arif and Wolfgang Frank

 

26.     High Frequency of Single-copy T-DNA Transformants Produced after Floral Dip in CRE-expressing Arabidopsis Plants

 

Annelies De Paepe, Sylvie De Buck, Jonah Nolf, and Ann Depicker

 

 

 

27.       A Developmentally Regulated Cre-lox system to Generate Marker-free Transgenic Brassica napus Plants

 

Lilya Kopertekh, Inge Broer, and Joachim Schiemann

 

 

 

28.       Exploiting Multisite Gateway and pENFRUIT Plasmid Collection for Fruit Genetic EngineeringLeandro H. Estornell, Antonio Granell, and Diego Orzaez

 

 

29.       A One-time Inducible Transposon to Create Knockout Mutants in Rice

 

Yuh-Chyang Charng

 

 

 

30.       Marker-free Gene Targeting by Recombinase-mediated Cassette Exchange (RMCE)

 

Hiroyasu Ebinuma, Kazuya Nanto, Saori Kasahara, and Atsushi Komamine

 

 

 

31.       Targeting DNA to a Previously Integrated Transgenic Locus Using Zinc Finger Nucleases

 

Tonya L. Strange Moynahan and Joseph F. Petolino

 

 

 

32.       Double-strand Break-induced Targeted Mutagenesis in Plants

 

L. Alexander Lyznik, Vesna Djukanovic, Meizhu Yang, and Spencer Jones

 

 

 

 

 

Part VII. Metabolic EnginEering and Pharming

 

33.       Combinatorial Genetic Transformation of Cereals and the Creation of Metabolic Libraries for the Carotenoid Pathway

 

Gemma Farre, Shaista Naqvi, Georgina Sanahuja, Chao Bai, Uxue Zorrilla-López, Sol M. Rivera, Ramon Canela, Gerhard Sandman, Richard M. Twyman, Teresa Capell, Changfu Zhu, and Paul Christou

 

 

 

34.       Production of a His-Tagged Canecystatin in Transgenic Sugarcane

 

Flavio Henrique-Silva and Andrea Soares-Costa

 

 

 

35.       Plastid Transformation as an Expression Tool for Plant-Derived Biopharmaceuticals

 

Nunzia Scotti and Teodoro Cardi

 

 

 

36.       Use of a Callus-Specific Selection System to Development Transgenic Rice Seed Accumulating a High Level of Recombinant Protein

 

Yuhya Wakasa and Fumio Takaiwa

 

 

 

Part VIII. Field TESTS

 

37.       How to Grow Transgenic Arabidopsis in the Field

 

Hanna Johansson Jänkänpää and Stefan Jansson

™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 

aids scientists in the continuous improvements being made for the production and analysis of transgenic plants.

Transgenic Plants: Methods and Protocols, Second Edition Methods in Molecular Biology Transgenic Plants: Methods and Protocols, Second Edition

Provides access to the most up-to-date improvements being made for the production and analysis of transgenic plants

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts



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