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RNA extraction for the assay of temporal gene expression in E. coli : A story of methodology development - Jas Wasniewski
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Jas Wasniewski:
RNA extraction for the assay of temporal gene expression in E. coli : A story of methodology development - Livres de poche

2012, ISBN: 3848437805

ID: 22510451363

[EAN: 9783848437801], Neubuch, [PU: LAP Lambert Academic Publishing Mrz 2012], This item is printed on demand - Print on Demand Neuware - Although gene expression studies have been performed on Escherichia coli using starvation models, there has been no study of long-term changes in gene expression due to the difficulty of isolating RNA of sufficient yield and quality at late time-points. Furthermore, there is evidence that these starvation models are not physiologically relevant compared to aging cells in batch culture. This document describes the development of a methodology for isolating RNA from aging and starving bacterial cells using organic extraction. Reverse-transcription quantitative PCR is then utilized to measure changes in gene expression for the purpose of corroborating previous microarray studies. Suggestions for isolating RNA from even older cultures are discussed as well as the limits of statistical analysis of gene expression in aging cell populations. A literature review of the small number of studies attempting to analyze starvation-induced and temporal gene expression in bacteria is also included. 96 pp. Englisch

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AHA-BUCH GmbH, Einbeck, Germany [51283250] [Rating: 5 (von 5)]
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RNA extraction for the assay of temporal gene expression in E. coli - Jas Wasniewski
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Jas Wasniewski:
RNA extraction for the assay of temporal gene expression in E. coli - Livres de poche

2012, ISBN: 3848437805

ID: 22512190156

[EAN: 9783848437801], Neubuch, [PU: LAP Lambert Academic Publishing Mrz 2012], Neuware - Although gene expression studies have been performed on Escherichia coli using starvation models, there has been no study of long-term changes in gene expression due to the difficulty of isolating RNA of sufficient yield and quality at late time-points. Furthermore, there is evidence that these starvation models are not physiologically relevant compared to aging cells in batch culture. This document describes the development of a methodology for isolating RNA from aging and starving bacterial cells using organic extraction. Reverse-transcription quantitative PCR is then utilized to measure changes in gene expression for the purpose of corroborating previous microarray studies. Suggestions for isolating RNA from even older cultures are discussed as well as the limits of statistical analysis of gene expression in aging cell populations. A literature review of the small number of studies attempting to analyze starvation-induced and temporal gene expression in bacteria is also included. 96 pp. Englisch

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Rheinberg-Buch, Bergisch Gladbach, Germany [53870650] [Rating: 5 (von 5)]
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RNA extraction for the assay of temporal gene expression in E. coli - Jas Wasniewski
Livre non disponible
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Jas Wasniewski:
RNA extraction for the assay of temporal gene expression in E. coli - Livres de poche

2012, ISBN: 3848437805

ID: 22512089373

[EAN: 9783848437801], Neubuch, [PU: LAP Lambert Academic Publishing Mrz 2012], Neuware - Although gene expression studies have been performed on Escherichia coli using starvation models, there has been no study of long-term changes in gene expression due to the difficulty of isolating RNA of sufficient yield and quality at late time-points. Furthermore, there is evidence that these starvation models are not physiologically relevant compared to aging cells in batch culture. This document describes the development of a methodology for isolating RNA from aging and starving bacterial cells using organic extraction. Reverse-transcription quantitative PCR is then utilized to measure changes in gene expression for the purpose of corroborating previous microarray studies. Suggestions for isolating RNA from even older cultures are discussed as well as the limits of statistical analysis of gene expression in aging cell populations. A literature review of the small number of studies attempting to analyze starvation-induced and temporal gene expression in bacteria is also included. 96 pp. Englisch

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RNA extraction for the assay of temporal gene expression in E. coli als Buch von Jas Wasniewski - LAP Lambert Academic Publishing
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RNA extraction for the assay of temporal gene expression in E. coli als Buch von Jas Wasniewski - nouveau livre

ISBN: 9783848437801

ID: 820005268

RNA extraction for the assay of temporal gene expression in E. coli ab 49 EURO A story of methodology development RNA extraction for the assay of temporal gene expression in E. coli ab 49 EURO A story of methodology development Bücher > Wissenschaft > Biologie

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RNA extraction for the assay of temporal gene expression in E. coli - Jas Wasniewski
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Jas Wasniewski:
RNA extraction for the assay of temporal gene expression in E. coli - Livres de poche

2012, ISBN: 3848437805

ID: 22412544661

[EAN: 9783848437801], Neubuch, [PU: LAP Lambert Academic Publishing], This item is printed on demand for shipment within 3 working days.

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EAN (ISBN-13): 9783848437801
ISBN (ISBN-10): 3848437805
Version reliée
Livre de poche
Date de parution: 2012
Editeur: AV Akademikerverlag GmbH & Co. KG.

Livre dans la base de données depuis 01.05.2008 12:03:30
Livre trouvé récemment le 01.09.2017 03:13:32
ISBN/EAN: 9783848437801

ISBN - Autres types d'écriture:
3-8484-3780-5, 978-3-8484-3780-1


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