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The Role of Lipid Rafts in Canine Distemper Virus Infection - Imhoff, Heidi
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Imhoff, Heidi:
The Role of Lipid Rafts in Canine Distemper Virus Infection - Livres de poche

2008, ISBN: 9783639046304

[ED: Taschenbuch / Paperback], [PU: VDM Verlag Dr. Müller], Cholesterol is known to play an important role in stabilisingparticular cellular membrane structures, so-called lipid rafts. Forseveral viruses a dependence on cholesterol for virus entry and/ormorphogenesis has been shown. Using flow cytometry and fluorescencemicroscopy we demonstrate that Canine distemper virus (CDV)infection of cells was not impaired after cellular cholesterol hadbeen depleted by the drug methyl-beta-cyclodextrin (MbCD). Thiseffect was independent of the multiplicity of infection (MOI) andthe cellular receptor used for infection. However, cholesteroldepletion of the viral envelope significantly reduced CDVinfectivity. Replenishment by addition of exogenous cholesterolrestored infectivity up to 80 percent. Thus, we conclude that CDVentry is dependent on cholesterol in the viral envelope.Furthermore, a reduced syncytium formation was observed when thecells were cholesterol depleted during the course of the infection.This may be related to the observation that the CDV envelopeproteins H and F partitioned into cellular detergent resistantmembranes (DRMs). Therefore, a role for lipid rafts during virusassembly and release as well is suggested., DE, [SC: 3.00], Neuware, gewerbliches Angebot, 220 mm, 176, [GW: 245g], Selbstabholung und Barzahlung, PayPal, offene Rechnung, Banküberweisung, Interntationaler Versand

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The Role of Lipid Rafts in Canine Distemper Virus Infection - Imhoff, Heidi
Livre non disponible
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Imhoff, Heidi:
The Role of Lipid Rafts in Canine Distemper Virus Infection - Livres de poche

2008, ISBN: 9783639046304

[ED: Taschenbuch / Paperback], [PU: VDM Verlag Dr. Müller], Cholesterol is known to play an important role in stabilisingparticular cellular membrane structures, so-called lipid rafts. Forseveral viruses a dependence on cholesterol for virus entry and/ormorphogenesis has been shown. Using flow cytometry and fluorescencemicroscopy we demonstrate that Canine distemper virus (CDV)infection of cells was not impaired after cellular cholesterol hadbeen depleted by the drug methyl-beta-cyclodextrin (MbCD). Thiseffect was independent of the multiplicity of infection (MOI) andthe cellular receptor used for infection. However, cholesteroldepletion of the viral envelope significantly reduced CDVinfectivity. Replenishment by addition of exogenous cholesterolrestored infectivity up to 80 percent. Thus, we conclude that CDVentry is dependent on cholesterol in the viral envelope.Furthermore, a reduced syncytium formation was observed when thecells were cholesterol depleted during the course of the infection.This may be related to the observation that the CDV envelopeproteins H and F partitioned into cellular detergent resistantmembranes (DRMs). Therefore, a role for lipid rafts during virusassembly and release as well is suggested., DE, [SC: 0.00], Neuware, gewerbliches Angebot, 220 mm, 176, [GW: 245g], Selbstabholung und Barzahlung, PayPal, offene Rechnung, Banküberweisung, Interntationaler Versand

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The Role of Lipid Rafts in Canine Distemper Virus Infection - Heidi Imhoff
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Heidi Imhoff:
The Role of Lipid Rafts in Canine Distemper Virus Infection - Livres de poche

ISBN: 9783639046304

[ED: Taschenbuch], [PU: VDM Verlag], Neuware - Cholesterol is known to play an important role in stabilisingparticular cellular membrane structures, so-called lipid rafts. Forseveral viruses a dependence on cholesterol for virus entry and/ormorphogenesis has been shown. Using flow cytometry and fluorescencemicroscopy we demonstrate that Canine distemper virus (CDV)infection of cells was not impaired after cellular cholesterol hadbeen depleted by the drug methyl-beta-cyclodextrin (MbCD). Thiseffect was independent of the multiplicity of infection (MOI) andthe cellular receptor used for infection. However, cholesteroldepletion of the viral envelope significantly reduced CDVinfectivity. Replenishment by addition of exogenous cholesterolrestored infectivity up to 80 percent. Thus, we conclude that CDVentry is dependent on cholesterol in the viral envelope.Furthermore, a reduced syncytium formation was observed when thecells were cholesterol depleted during the course of the infection.This may be related to the observation that the CDV envelopeproteins H and F partitioned into cellular detergent resistantmembranes (DRMs). Therefore, a role for lipid rafts during virusassembly and release as well is suggested., DE, [SC: 0.00], Neuware, gewerbliches Angebot, 220x150x11 mm, 176, [GW: 278g], PayPal, offene Rechnung, Banküberweisung, Interntationaler Versand

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The Role of Lipid Rafts in Canine Distemper Virus Infection - Heidi Imhoff
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Heidi Imhoff:
The Role of Lipid Rafts in Canine Distemper Virus Infection - nouveau livre

ISBN: 9783639046304

ID: 1ffe0b42ac9785d2e17dfaef37ea607b

Cholesterol is known to play an important role in stabilisingparticular cellular membrane structures, so-called lipid rafts. Forseveral viruses a dependence on cholesterol for virus entry and/ormorphogenesis has been shown. Using flow cytometry and fluorescencemicroscopy we demonstrate that Canine distemper virus (CDV)infection of cells was not impaired after cellular cholesterol hadbeen depleted by the drug methyl-beta-cyclodextrin (MbCD). Thiseffect was independent of the multiplicity of infection (MOI) andthe cellular receptor used for infection. However, cholesteroldepletion of the viral envelope significantly reduced CDVinfectivity. Replenishment by addition of exogenous cholesterolrestored infectivity up to 80 percent. Thus, we conclude that CDVentry is dependent on cholesterol in the viral envelope.Furthermore, a reduced syncytium formation was observed when thecells were cholesterol depleted during the course of the infection.This may be related to the observation that the CDV envelopeproteins H and F partitioned into cellular detergent resistantmembranes (DRMs). Therefore, a role for lipid rafts during virusassembly and release as well is suggested. Bücher / Naturwissenschaften, Medizin, Informatik & Technik / Biologie, [PU: VDM Verlag Dr. Müller, Saarbrücken]

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The Role of Lipid Rafts in Canine Distemper Virus Infection - Heidi Imhoff
Livre non disponible
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Heidi Imhoff:
The Role of Lipid Rafts in Canine Distemper Virus Infection - nouveau livre

2008, ISBN: 9783639046304

ID: 9642828

unbekannt, Buch, [PU: VDM Verlag Dr. Müller]

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Détails sur le livre
The Role of Lipid Rafts in Canine Distemper Virus Infection

Cholesterol is known to play an important role in stabilising particular cellular membrane structures, so-called lipid rafts. For several viruses a dependence on cholesterol for virus entry and/or morphogenesis has been shown. Using flow cytometry and fluorescence microscopy we demonstrate that Canine distemper virus (CDV) infection of cells was not impaired after cellular cholesterol had been depleted by the drug methyl-beta-cyclodextrin (MbCD). This effect was independent of the multiplicity of infection (MOI) and the cellular receptor used for infection. However, cholesterol depletion of the viral envelope significantly reduced CDV infectivity. Replenishment by addition of exogenous cholesterol restored infectivity up to 80 percent. Thus, we conclude that CDV entry is dependent on cholesterol in the viral envelope. Furthermore, a reduced syncytium formation was observed when the cells were cholesterol depleted during the course of the infection. This may be related to the observation that the CDV envelope proteins H and F partitioned into cellular detergent resistant membranes (DRMs). Therefore, a role for lipid rafts during virus assembly and release as well is suggested.

Informations détaillées sur le livre - The Role of Lipid Rafts in Canine Distemper Virus Infection


EAN (ISBN-13): 9783639046304
ISBN (ISBN-10): 3639046307
Livre de poche
Date de parution: 2008
Editeur: VDM Verlag
176 Pages
Poids: 0,278 kg
Langue: eng/Englisch

Livre dans la base de données depuis 17.01.2008 10:57:09
Livre trouvé récemment le 20.08.2017 08:25:47
ISBN/EAN: 9783639046304

ISBN - Autres types d'écriture:
3-639-04630-7, 978-3-639-04630-4


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