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In recent years, our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerat… Plus…

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ISBN: 9781617796630

In recent years, our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerat… Plus…

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Epidermal Cells - Livres de poche

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[EAN: 9781617796630], Neubuch, [PU: Humana Press], DNA ENDOPLASMATISCHESRETICULUM FISCHE MICROARRAY PRECURSORS PROGENITORS PROTEOMICS VIVO GENEEXPRESSION GENES HYBRIDIZATION MOLECULARBIOL… Plus…

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[ED: Kartoniert / Broschiert], [PU: Humana Press], Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Covers numerous state-of-the-art approa… Plus…

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Informations détaillées sur le livre - Epidermal Cells


EAN (ISBN-13): 9781617796630
ISBN (ISBN-10): 1617796638
Version reliée
Livre de poche
Date de parution: 2014
Editeur: Springer
464 Pages
Poids: 0,958 kg
Langue: Englisch

Livre dans la base de données depuis 2008-12-15T02:26:34+01:00 (Paris)
Page de détail modifiée en dernier sur 2022-02-06T00:51:13+01:00 (Paris)
ISBN/EAN: 9781617796630

ISBN - Autres types d'écriture:
1-61779-663-8, 978-1-61779-663-0


Données de l'éditeur

Auteur: Kursad Turksen
Titre: Methods in Molecular Biology; Epidermal Cells - Methods and Protocols
Editeur: Humana; Humana Press
446 Pages
Date de parution: 2012-03-07
Totowa; NJ; US
Imprimé / Fabriqué en
Langue: Anglais
155,14 € (DE)
159,49 € (AT)
171,50 CHF (CH)
POD
XVI, 446 p. 85 illus., 29 illus. in color.

BC; Hardcover, Softcover / Biologie/Mikrobiologie; Zellbiologie (Zytologie); Verstehen; DNA; Endoplasmatisches Reticulum; Fische; Microarray; Precursors; Progenitors; Proteomics; Vivo; gene expression; genes; hybridization; molecular biology; reproducible techniques; tissue; transcription; Cell culture; Cell Biology; Cytological Techniques; Wissenschaftliche Ausstattung, Experimente und Techniken; BB

Isolation of Adult Mouse Stem Keratinocytes Using Magnetic Cell Sorting (MACS).- Functional Investigations of Keratinocyte Stem Cells and Progenitors at a Single-Cell Level Using Multiparallel Clonal Microcultures.- Growth and Stratification of Epithelial Cells in Minimal Culture Conditions.- Matched Cultures of Keratinocytes and Fibroblasts Derived from Normal and NER-Deficient Mouse Models.- Establishment of Spontaneously Immortalized Keratinocyte Lines from Wild-Type and Mutant Mice.- Study of Epidermal Differentiation in Human Keratinocytes Cultured in Autocrine Conditions.- Directed Differentiation of Human Embryonic Stem Cells to Epidermal Progenitors.- Expression and Analysis of Exogenous Proteins in Epidermal Cells.- Using siRNA Knockdown in HaCaT Cells to Study Transcriptional Control of Epidermal Proliferation Potential.- RNA Interference in Keratinocytes and an Organotypic Model of Human Epidermis.- Scanning for Transcription Factor Binding by a Variant EMSA.- Chromatin Immunoprecipitation for Identifying Transcription Factor Targets in Keratinocytes.- Gene Expression Profiling of Mouse Epidermal Keratinocytes.- Analysis of Tissue-Specific Gene Expression Using Laser Capture Microdissection.- Comprehensive Transcriptional Profiling of Human Epidermis, Reconstituted Epidermal Equivalents, and Cultured Keratinocytes Using DNA Microarray Chips.- Molecular Profiling of the Epidermis: A Proteomics Approach.- Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridization.- Embryonic Mammary Anlagen Analysis Using Immunolabelling of Whole Mounts.- Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis.- Tetracycline-Regulated Gene Expression in Transgenic Mouse Epidermis.- A Versatile Murine 3D Organotypic Model to Evaluate Aspects of Wound Healing and Epidermal Organization.- Optical and Biochemical Dissection of Connexin and Disease-Linked Connexin Mutants in 3D Organotypic Epidermis.- Cytokine Release in Tissue-Engineered Epidermal Equivalents After Prolonged Mechanical Loading.- Three-Dimensional Human Tissue Models of Wounded Skin.- In Vivo Transplantation of Genetically Modified Mouse Embryonic Epidermis.- A Transplant Model for Human Epidermal Skin Regeneration.- Identification of Epithelial Stem Cells In Vivo and In Vitro Using Keratin 19 and BrdU.- Isolation and Culture of Hair Follicle Pluripotent Stem (hfPS) Cells and Their Use for Nerve and Spinal Cord Regeneration.- Limiting Dilution Analysis of Murine Epidermal Stem Cells Using an In Vivo Regeneration Assay.
Covers numerous state-of-the-art approaches to analyze epidermal differentiation Provides step by step reproducible protocols for novices and experienced investigators Comprises a comprehensive collection of methods in one source Covers most recent developments in epidermal cell methodologies Includes supplementary material: sn.pub/extras

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