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Methods in Molecular Biology: Reverse Chemical Genetics - Methods and Protocols - Koga, Hisashi (Hrsg.)
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Koga, Hisashi (Hrsg.):

Methods in Molecular Biology: Reverse Chemical Genetics - Methods and Protocols - Livres de poche

2014, ISBN: 9781617796418

[ED: Taschenbuch / Paperback], [PU: Springer, Berlin], KURZE BESCHREIBUNG/ANMERKUNGEN: This book orchestrates the differential fields of technology in order to create a new era of the study of reverse chemical genetics. It introduces fundamental resources as well as the modification of these resources by recent molecular technologies. AUSFÜHRLICHERE BESCHREIBUNG: Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established methods in addition to potentially applicable techniques that make use of specific small molecules, plus functional assays utilizing these molecules, while focusing on methods that make feasible the functional discovery and comprehensive knowledge of particular target genes. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Reverse Chemical Genetics: Methods and Protocols promises to inspire researchers to conduct their own experiments in order to progress this vital field of study. INHALT: Part I: The Importance of Fully-Characterized Genomic Resources and Informatics for Reverse Chemical Genetics 1. ORFeome Cloning Osamu Ohara 2. Systematic Cloning of an ORFeome Using the Gateway System Akihisa Matsuyama and Minoru Yoshida 3. High-Throughput Construction of ORF Clones for Production of the Recombinant Proteins Hisashi Yamakawa 4. Computational Overview of GPCR Gene Universe to Support Reverse Chemical Genomics Study Makiko Suwa and Yukiteru Ono 5. High-Performance Gene Expression Module Analysis Tool and Its Application to Chemical Toxicity Data Wataru Fujibuchi, Hyeryung Kim, Yoshifumi Okada, Takeaki Taniguchi, and Hideko Sone 6. Human Protein Reference Database and Human Proteinpedia as Discovery Tools for Systems Biology T.S. Keshava Prasad, Kumaran Kandasamy, and Akhilesh Pandey Part II: Modification of cDNA Resources Utilized for Reverse Chemical Genetics 7. High-Throughput Production of the Recombinant Proteins Expressed in Escherichia coli Utilizing cDNA Resources Kiyo Shimada and Hisashi Koga 8. An Insect Cell-Free System for Recombinant Protein Expression Using cDNA Resources Takashi Suzuki, Toru Ezure, Masaaki Ito, Masamitsu Shikata, and Eiji Ando 9. Recombinant Protein Production by a Kaiko-Baculovirus System Hidekazu Nagaya 10. Pulse-Chase Experiment for the Analysis of Protein Stability in Cultured Mammalian Cells by Covalent Fluorescent Labeling of Fusion Proteins Kei Yamaguchi, Shinichi Inoue, Osamu Ohara, and Takahiro Nagase Part III: The Refinement of Established Methods by Recent Innovations 11. Application of 2D-DIGE in Cancer Proteomics toward Personalized Medicine Tadashi Kondo and Setsuo Hirohashi 12. Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein AnalysisAtsunori Hiratsuka and Kenji Yokoyama 13. Large-Scale Whole Mount In Situ Hybridization of Mouse Embryos Hirohito Shimizu, Kenta Uchibe, and Hiroshi Asahara 14. Identification of the Specific Binding Proteins of Bioactive Small Compound Using Affinity Resins Akito Tanaka Part IV: Potential Applicable Techniques for Reverse Chemical Genetics 15. Protein Microarrays: Effective Tools for the Study of Inflammatory Diseases Xiaobo Yu, Nicole Schneiderhan-Marra, Hsin-Yun Hsu, Jutta Bachmann, and Thomas O. Joos 16. Preparation of Highly Sensitive Protein Array Using Reactive Polymer Toshifumi Shiroya, Hiroyuki Tanaka, Minako Hanasaki, and Hisao Takeuchi 17. High-Throughput SPR Biosensor Motoki Kyo, Kimihiko Ohtsuka, Eiji Okamoto, and Kazuki Inamori 18. Drug Discovery through Functional Screening in the Drosophila Heart Takeshi Akasaka and Karen Ocorr 19. Permeable Cell Assay: A Method for High-Throughput Measurement of Cellular ATP Synthetic Activity Kiyotaka Y. Hara 20. Novel Methodology for Immobilization of Biomolecules on the Surface of a Photo-Responsible Polymer Containing Azobenzene Moiety Osamu Watanabe, [SC: 0.00]

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Methods in Molecular Biology: Reverse Chemical Genetics - Methods and Protocols - Koga, Hisashi (Hrsg.)
Livre non disponible
(*)

Koga, Hisashi (Hrsg.):

Methods in Molecular Biology: Reverse Chemical Genetics - Methods and Protocols - Livres de poche

2014, ISBN: 9781617796418

[ED: Taschenbuch / Paperback], [PU: Springer, Berlin], KURZE BESCHREIBUNG/ANMERKUNGEN: This book orchestrates the differential fields of technology in order to create a new era of the study of reverse chemical genetics. It introduces fundamental resources as well as the modification of these resources by recent molecular technologies. AUSFÜHRLICHERE BESCHREIBUNG: Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established methods in addition to potentially applicable techniques that make use of specific small molecules, plus functional assays utilizing these molecules, while focusing on methods that make feasible the functional discovery and comprehensive knowledge of particular target genes. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Reverse Chemical Genetics: Methods and Protocols promises to inspire researchers to conduct their own experiments in order to progress this vital field of study. INHALT: Part I: The Importance of Fully-Characterized Genomic Resources and Informatics for Reverse Chemical Genetics1. ORFeome CloningOsamu Ohara2. Systematic Cloning of an ORFeome Using the Gateway SystemAkihisa Matsuyama and Minoru Yoshida3. High-Throughput Construction of ORF Clones for Production of the Recombinant ProteinsHisashi Yamakawa4. Computational Overview of GPCR Gene Universe to Support Reverse Chemical Genomics Study Makiko Suwa and Yukiteru Ono5. High-Performance Gene Expression Module Analysis Tool and Its Application to Chemical Toxicity DataWataru Fujibuchi, Hyeryung Kim, Yoshifumi Okada, Takeaki Taniguchi, and Hideko Sone6. Human Protein Reference Database and Human Proteinpedia as Discovery Tools for Systems BiologyT.S. Keshava Prasad, Kumaran Kandasamy, and Akhilesh PandeyPart II: Modification of cDNA Resources Utilized for Reverse Chemical Genetics7. High-Throughput Production of the Recombinant Proteins Expressed in Escherichia coli Utilizing cDNA ResourcesKiyo Shimada and Hisashi Koga8. An Insect Cell-Free System for Recombinant Protein Expression Using cDNA ResourcesTakashi Suzuki, Toru Ezure, Masaaki Ito, Masamitsu Shikata, and Eiji Ando9. Recombinant Protein Production by a Kaiko-Baculovirus SystemHidekazu Nagaya10. Pulse-Chase Experiment for the Analysis of Protein Stability in Cultured Mammalian Cells by Covalent Fluorescent Labeling of Fusion ProteinsKei Yamaguchi, Shinichi Inoue, Osamu Ohara, and Takahiro NagasePart III: The Refinement of Established Methods by Recent Innovations11. Application of 2D-DIGE in Cancer Proteomics toward Personalized MedicineTadashi Kondo and Setsuo Hirohashi12. Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein AnalysisAtsunori Hiratsuka and Kenji Yokoyama13. Large-Scale Whole Mount In Situ Hybridization of Mouse EmbryosHirohito Shimizu, Kenta Uchibe, and Hiroshi Asahara14. Identification of the Specific Binding Proteins of Bioactive Small Compound Using Affinity ResinsAkito TanakaPart IV: Potential Applicable Techniques for Reverse Chemical Genetics15. Protein Microarrays: Effective Tools for the Study of Inflammatory DiseasesXiaobo Yu, Nicole Schneiderhan-Marra, Hsin-Yun Hsu, Jutta Bachmann, and Thomas O. Joos16. Preparation of Highly Sensitive Protein Array Using Reactive PolymerToshifumi Shiroya, Hiroyuki Tanaka, Minako Hanasaki, and Hisao Takeuchi17. High-Throughput SPR BiosensorMotoki Kyo, Kimihiko Ohtsuka, Eiji Okamoto, and Kazuki Inamori18. Drug Discovery through Functional Screening in the Drosophila HeartTakeshi Akasaka and Karen Ocorr19. Permeable Cell Assay: A Method for High-Throughput Measurement of Cellular ATP Synthetic ActivityKiyotaka Y. Hara20. Novel Methodology for Immobilization of Biomolecules on the Surface of a Photo-Responsible Polymer Containing Azobenzene MoietyOsamu Watanabe, [SC: 0.00]

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Reverse Chemical Genetics - Koga, Hisashi
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Koga, Hisashi:
Reverse Chemical Genetics - nouveau livre

ISBN: 1617796417

ID: 7626726406

[EAN: 9781617796418], Neubuch, Publisher/Verlag: Springer, Berlin | Methods and Protocols | This book orchestrates the differential fields of technology in order to create a new era of the study of reverse chemical genetics. It introduces fundamental resources as well as the modification of these resources by recent molecular technologies. | Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established methods in addition to potentially applicable techniques that make use of specific small molecules, plus functional assays utilizing these molecules, while focusing on methods that make feasible the functional discovery and comprehensive knowledge of particular target genes. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Reverse Chemical Genetics: Methods and Protocols promises to inspire researchers to conduct their own experiments in order to progress this vital field of study. | Part I: The Importance of Fully-Characterized Genomic Resources and Informatics for Reverse Chemical Genetics 1. ORFeome Cloning Osamu Ohara 2. Systematic Cloning of an ORFeome Using the Gateway System Akihisa Matsuyama and Minoru Yoshida 3. High-Throughput Construction of ORF Clones for Production of the Recombinant Proteins Hisashi Yamakawa 4. Computational Overview of GPCR Gene Universe to Support Reverse Chemical Genomics Study Makiko Suwa and Yukiteru Ono 5. High-Performance Gene Expression Module Analysis Tool and Its Application to Chemical Toxicity Data Wataru Fujibuchi, Hyeryung Kim, Yoshifumi Okada, Takeaki Taniguchi, and Hideko Sone 6. Human Protein Reference Database and Human Proteinpedia as Discovery Tools for Systems Biology T.S. Keshava Prasad, Kumaran Kandasamy, and Akhilesh Pandey Part II: Modification of cDNA Resources Utilized for Reverse Chemical Genetics 7. High-Throughput Production of the Recombinant Proteins Expressed in Escherichia coli Utilizing cDNA Resources Kiyo Shimada and Hisashi Koga 8. An Insect Cell-Free System for Recombinant Protein Expression Using cDNA Resources Takashi Suzuki, Toru Ezure, Masaaki Ito, Masamitsu Shikata, and Eiji Ando 9. Recombinant Protein Production by a Kaiko-Baculovirus System Hidekazu Nagaya 10. Pulse-Chase Experiment for the Analysis of Protein Stability in Cultured Mammalian Cells by Covalent Fluorescent Labeling of Fusion Proteins Kei Yamaguchi, Shinichi Inoue, Osamu Ohara, and Takahiro Nagase Part III: The Refinement of Established Methods by Recent Innovations 11. Application of 2D-DIGE in Cancer Proteomics toward Personalized Medicine Tadashi Kondo and Setsuo Hirohashi 12. Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein Analysis Atsunori Hiratsuka and Kenji Yokoyama 13. Large-Scale Whole Mount In Situ Hybridization of Mouse Embryos Hirohito Shimizu, Kenta Uchibe, and Hiroshi Asahara 14. Identification of the Specific Binding Proteins of Bioactive Small Compound Using Affinity Resins Akito Tanaka Part IV: Potential Applicable Techniques for Reverse Chemical Genetics 15. Protein Microarrays: Effective Tools for the Study of Inflammatory Diseases Xiaobo Yu, Nicole Schneiderhan-Marra, Hsin-Yun Hsu, Jutta Bachmann, and Thomas O. Joos 16. Preparation of Highly Sensitive Protein Array Using Reactive Polymer Toshifumi Shiroya, Hiroyuki Tanaka, Minako Hanasaki, and Hisao Takeuchi 17. High-T

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Reverse Chemical Genetics - Humana Press
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Humana Press:
Reverse Chemical Genetics - nouveau livre

ISBN: 9781617796418

ID: 142457697

Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established methods in addition to potentially applicable techniques that make use of specific small molecules, plus functional assays utilizing these molecules, while focusing on methods that make feasible the functional discovery and comprehensive knowledge of particular target genes. As a volume in the highly successful Methods in Molecular Biology(TM) series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Reverse Chemical Genetics: Methods and Protocols promises to inspire researchers to conduct their own experiments in order to progress this vital field of study. This book orchestrates the differential fields of technology in order to create a new era of the study of reverse chemical genetics. It introduces fundamental resources as well as the modification of these resources by recent molecular technologies. Buch (fremdspr.) Bücher>Fremdsprachige Bücher>Englische Bücher, Humana Press

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Reverse Chemical Genetics - Hisashi Koga
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Hisashi Koga:
Reverse Chemical Genetics - nouveau livre

ISBN: 1617796417

ID: 9781617796418

, [Kein Buch] <-> Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established methods in addition to potentially applicable techniques that make use of specific small molecules, plus functional assays utilizing these molecules, while focusing on methods that make feasible the functional discovery and comprehensive knowledge of particular target genes. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Reverse Chemical Genetics: Methods and Protocols promises to inspire researchers to conduct their own experiments in order to progress this vital field of study. <-> HC/Medizin/Ganzheitsmedizin

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Détails sur le livre
Reverse Chemical Genetics
Auteur:

Hisashi Koga

Titre:

Reverse Chemical Genetics

ISBN:

9781617796418

Informations détaillées sur le livre - Reverse Chemical Genetics


EAN (ISBN-13): 9781617796418
ISBN (ISBN-10): 1617796417
Livre de poche
Date de parution: 2014
Editeur: Humana Press
284 Pages
Poids: 0,596 kg
Langue: Englisch

Livre dans la base de données depuis 19.07.2007 13:14:31
Livre trouvé récemment le 06.07.2016 23:26:51
ISBN/EAN: 9781617796418

ISBN - Autres types d'écriture:
1-61779-641-7, 978-1-61779-641-8

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