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Methods in Gut Microbial Ecology for Ruminants - edition reliée, livre de poche

2005

ISBN: 1402037902

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Methods In Gut Microbial Ecology For Ruminants by Harinder P.s. Makkar Hardcover | Indigo Chapters

This book presents a comprehensive up-to-date account of the methodologies and protocols for conventional and modern molecular techniques that are currently in use for studying the gut microbial ecology of ruminants. Each chapter has been contributed by experts in the field and methods have been presented in a recipe-like format designed for direct practical use in the laboratory and also to provide insight into the most appropriate techniques, their applications and the type of information that could be expected. The techniques and procedures described are also relevant and adaptable to other gastrointestinal ecosystems and the microbiology of anaerobic environments in general. This manual will 'demystify' the methods in molecular microbial ecology for readers who are novice in the field but are excited by the prospects of the technology. It would also be invaluable for the experienced workers striving for giving new dimension to their research - expanding the work in other fields and initiating cross-cutting activities.

Informations détaillées sur le livre - Methods In Gut Microbial Ecology For Ruminants by Harinder P.s. Makkar Hardcover | Indigo Chapters


EAN (ISBN-13): 9781402037900
ISBN (ISBN-10): 1402037902
Version reliée
Date de parution: 2005
Editeur: Harinder P.s. Makkar
225 Pages
Poids: 0,523 kg
Langue: eng/Englisch

Livre dans la base de données depuis 2007-05-14T06:31:09+02:00 (Paris)
Page de détail modifiée en dernier sur 2023-11-09T18:27:56+01:00 (Paris)
ISBN/EAN: 9781402037900

ISBN - Autres types d'écriture:
1-4020-3790-2, 978-1-4020-3790-0
Autres types d'écriture et termes associés:
Auteur du livre: gagern, christopher
Titre du livre: methods gut microbial ecology for ruminants, bavariapark, rumi, sattler


Données de l'éditeur

Auteur: Harinder P.S. Makkar; Christopher S. McSweeney
Titre: Methods in Gut Microbial Ecology for Ruminants
Editeur: Springer; Springer Netherland
226 Pages
Date de parution: 2005-11-03
Dordrecht; NL
Langue: Anglais
213,99 € (DE)
219,99 € (AT)
236,00 CHF (CH)
Available
XII, 226 p.

BB; Hardcover, Softcover / Biologie/Zoologie; Zoologie und Tierwissenschaften; Verstehen; Biome; Ecology; Expression; FISH; Nucleotide; PCR; gene expression; genes; hybridization; microorganisms; rDNA; ribosomal RNA; Zoology; Agriculture; Ecology; Microbial Ecology; Molecular Ecology; Microbiology; Agrarwissenschaften; Ökologie, Biosphäre; Mikrobiologie (nicht-medizinisch); Molekularbiologie; BC

Foreword; Introduction; Editorial Note; Part 1. Designing in-vivo Microbial Ecology Studies; 1.1. Experimental designs for rumen microbiology, Adrian R. Egan; Part 2. Classical Methods for Isolation, Enumeration, Cultivation and Functional Assays of Rumen Microbes; 2.1. Rumen bacteria, Christopher S. McSweeney, Stuart E. Denman and Roderick I. Mackie; 2.2. Bacteriophages, Athol V. Klieve. 2.3. Methanogenic archaea, Keith N. Joblin; 2.4. Anaerobic fungi, Michael K. Theodorou, Jayne Brookman and Anthony P.J. Trinci; 2.5. Ciliate protozoa, Burk A. Dehority; Part 3. PCR-Based Methods for Analysis of Populations and Gene Expression; 3.1. Nucleic acid extraction, oligonucleotide probes and PCR methods, Zhongtang Yu and Robert J. Forster; 3.2. Quantitative (real-time) PCR, Stuart E. Denman and Christopher S. McSweeney; Part 4. Molecular Fingerprinting Techniques for Genotypic Analysis of Pure Cultures and Microbial Communities; 4.1. Denaturing gradient gel electrophoresis, Svetlana A. Kocherginskaya, Isaac K.O. Cann and Roderick I. Mackie; 4.2. Bacteriophage populations, Athol V. Klieve and Rosalind A. Gilbert; 4.3. Anaerobic fungal populations, Jayne L. Brookman and Matthew J. Nicholson; 4.4. RAPD, RFLP, T-RFLP, AFLP, RISA, Stuart E. Denman, Makoto Mitsumori and Christopher S. McSweeney; Part 5. DNA Clone Libraries of Microbial Communities; 5.1. 16S/18S ribosomal DNA clone library analysis of rumen microbial diversity, André-Denis G. Wright, Kiyoshi Tajima and Rustam I. Aminov; Part 6. Use of Small Subunit Ribosomal RNA Directed Oligonucleotide Probes for Microbial Population Studies; 6.1. Northern blot analysis to investigate the abundance of micro-organisms, Denis O. Krause; 6.2. Whole cell probing with fluorescently labelled probes for in situ analysis of microbial populations, Linda L. Blackall; 6.3. Combined fluorescence in situ hybridization and microautoradiography (FISH-MAR), Maneesha P. Ginige; Part 7. Genomic Analysis of Microbial Ecosystems; 7.1.Metagenomic analysis of the microbiomes in ruminants and other herbivores Mark Morrison, Sarah E. Adams, Karen E. Nelson and Graeme T. Attwood; Chapterwise Keywords; Keyword Index.


A comprehensive up-to-date account of the methodologies and protocols for conventional and modern molecular techniques, currently in use for studying the gut microbial ecology of ruminants Written by an expert group of scientists working in the area of ruminant digestion and gut microbiology Written in a recipe-like format designed for direct practical use in the laboratory Only book in the market presenting recent molecular methodologies for studying gut microbial ecology in ruminants

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