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POLYMER AND QUANTUM DOTS FOR IMMUNOBASED DETECTION OF S.TYPHI IN WATER - Polymeric membrane for Ab immobilization and bacteria capturing. Immunocomplex detection by HRP and QDs labeled Ab. - Jackeray, Richa
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Jackeray, Richa:
POLYMER AND QUANTUM DOTS FOR IMMUNOBASED DETECTION OF S.TYPHI IN WATER - Polymeric membrane for Ab immobilization and bacteria capturing. Immunocomplex detection by HRP and QDs labeled Ab. - Livres de poche

2011, ISBN: 9783844399295

[ED: Taschenbuch / Paperback], [PU: Dictus Publishing], This thesis is devoted towards the development of polymeric matrices and bioconjugated quantum dots for immuno-based detection of S.typhi in water. Thesis involves the surface modification of nylon membrane by grafting with GMA using free radical graft copolymerization. S.typhi specific IgG immobilized NyM-g-GMA was used as solid phase in development of ELISA for detection of S.typhi. Developed sandwich ELISA was found to be specific, reproducible, reasonably rapid and sensitive with detection limit of 103cells/mL. Developed sandwich ELISA was found to have two-fold higher limit of detection as compared to the assay performed using PS plates. For further validation of the developed immunoassay, water samples were collected from different sources in and around Delhi and tested for various parameters of validation. A method for fluorescence based detection of sandwich immunocomplex of bacteria and antibody conjugated CdSe/ZnS quantum dots on surface modified porous polycarbonate membrane was also developed. Fluorescent aggregates were observed on the anti-Sty IgG immobilized membranes under confocal microscope and semi-quantified using spectral profiling., DE, [SC: 0.00], Neuware, gewerbliches Angebot, 220, Selbstabholung und Barzahlung, PayPal, offene Rechnung, Banküberweisung, Interntationaler Versand

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POLYMER AND QUANTUM DOTS FOR IMMUNOBASED DETECTION OF S.TYPHI IN WATER - Richa Jackeray
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Richa Jackeray:
POLYMER AND QUANTUM DOTS FOR IMMUNOBASED DETECTION OF S.TYPHI IN WATER - Livres de poche

2011, ISBN: 3844399291

ID: 10409413837

[EAN: 9783844399295], Neubuch, [PU: LAP Lambert Acad. Publ. Mai 2011], This item is printed on demand - Print on Demand Neuware - This thesis is devoted towards the development of polymeric matrices and bioconjugated quantum dots for immuno-based detection of S.typhi in water. Thesis involves the surface modification of nylon membrane by grafting with GMA using free radical graft copolymerization. S.typhi specific IgG immobilized NyM-g-GMA was used as solid phase in development of ELISA for detection of S.typhi. Developed sandwich ELISA was found to be specific, reproducible, reasonably rapid and sensitive with detection limit of 103cells/mL. Developed sandwich ELISA was found to have two-fold higher limit of detection as compared to the assay performed using PS plates. For further validation of the developed immunoassay, water samples were collected from different sources in and around Delhi and tested for various parameters of validation. A method for fluorescence based detection of sandwich immunocomplex of bacteria and antibody conjugated CdSe/ZnS quantum dots on surface modified porous polycarbonate membrane was also developed. Fluorescent aggregates were observed on the anti-Sty IgG immobilized membranes under confocal microscope and semi-quantified using spectral profiling. 220 pp. Englisch

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POLYMER AND QUANTUM DOTS FOR IMMUNOBASED DETECTION OF S.TYPHI IN WATER - Richa Jackeray
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ISBN: 9783844399295

ID: 93eac88bb255a0e94cee2930268b3608

This thesis is devoted towards the development of polymeric matrices and bioconjugated quantum dots for immuno-based detection of S.typhi in water. Thesis involves the surface modification of nylon membrane by grafting with GMA using free radical graft copolymerization. S.typhi specific IgG immobilized NyM-g-GMA was used as solid phase in development of ELISA for detection of S.typhi. Developed sandwich ELISA was found to be specific, reproducible, reasonably rapid and sensitive with detection limit of 103cells/mL. Developed sandwich ELISA was found to have two-fold higher limit of detection as compared to the assay performed using PS plates. For further validation of the developed immunoassay, water samples were collected from different sources in and around Delhi and tested for various parameters of validation. A method for fluorescence based detection of sandwich immunocomplex of bacteria and antibody conjugated CdSe/ZnS quantum dots on surface modified porous polycarbonate membrane was also developed. Fluorescent aggregates were observed on the anti-Sty IgG immobilized membranes under confocal microscope and semi-quantified using spectral profiling. Bücher / Naturwissenschaften, Medizin, Informatik & Technik / Technik / Chemische Technik

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POLYMER AND QUANTUM DOTS FOR IMMUNOBASED DETECTION OF S.TYPHI IN WATER - Jackeray, Richa
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Jackeray, Richa:
POLYMER AND QUANTUM DOTS FOR IMMUNOBASED DETECTION OF S.TYPHI IN WATER - Livres de poche

2011, ISBN: 9783844399295

[ED: Softcover], [PU: Dictus Publishing], This thesis is devoted towards the development of polymeric matrices and bioconjugated quantum dots for immuno-based detection of S.typhi in water. Thesis involves the surface modification of nylon membrane by grafting with GMA using free radical graft copolymerization. S.typhi specific IgG immobilized NyM-g-GMA was used as solid phase in development of ELISA for detection of S.typhi. Developed sandwich ELISA was found to be specific, reproducible, reasonably rapid and sensitive with detection limit of 103cells/mL. Developed sandwich ELISA was found to have two-fold higher limit of detection as compared to the assay performed using PS plates. For further validation of the developed immunoassay, water samples were collected from different sources in and around Delhi and tested for various parameters of validation. A method for fluorescence based detection of sandwich immunocomplex of bacteria and antibody conjugated CdSe/ZnS quantum dots on surface modified porous polycarbonate membrane was also developed. Fluorescent aggregates were observed on the anti-Sty IgG immobilized membranes under confocal microscope and semi-quantified using spectral profiling.2011. 220 S.Versandfertig in 3-5 Tagen

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POLYMER AND QUANTUM DOTS FOR IMMUNOBASED DETECTION OF S.TYPHI IN WATER - Jackeray, Richa
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Jackeray, Richa:
POLYMER AND QUANTUM DOTS FOR IMMUNOBASED DETECTION OF S.TYPHI IN WATER - Livres de poche

2011, ISBN: 3844399291

Edition reliée, ID: 10619883

Polymeric membrane for Ab immobilization and bacteria capturing. Immunocomplex detection by HRP and QDs labeled Ab. - Buch, gebundene Ausgabe, 220 S., Beilagen: Paperback, Erschienen: 2011 LAP Lambert Acad. Publ.

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POLYMER AND QUANTUM DOTS FOR IMMUNOBASED DETECTION OF S.TYPHI IN WATER

This thesis is devoted towards the development of polymeric matrices and bioconjugated quantum dots for immuno-based detection of S.typhi in water. Thesis involves the surface modification of nylon membrane by grafting with GMA using free radical graft copolymerization. S.typhi specific IgG immobilized NyM-g-GMA was used as solid phase in development of ELISA for detection of S.typhi. Developed sandwich ELISA was found to be specific, reproducible, reasonably rapid and sensitive with detection limit of 103cells/mL. Developed sandwich ELISA was found to have two-fold higher limit of detection as compared to the assay performed using PS plates. For further validation of the developed immunoassay, water samples were collected from different sources in and around Delhi and tested for various parameters of validation. A method for fluorescence based detection of sandwich immunocomplex of bacteria and antibody conjugated CdSe/ZnS quantum dots on surface modified porous polycarbonate membrane was also developed. Fluorescent aggregates were observed on the anti-Sty IgG immobilized membranes under confocal microscope and semi-quantified using spectral profiling.

Informations détaillées sur le livre - POLYMER AND QUANTUM DOTS FOR IMMUNOBASED DETECTION OF S.TYPHI IN WATER


EAN (ISBN-13): 9783844399295
ISBN (ISBN-10): 3844399291
Version reliée
Livre de poche
Date de parution: 2011
Editeur: LAP Lambert Acad. Publ. Mai 2011

Livre dans la base de données depuis 19.05.2008 03:45:38
Livre trouvé récemment le 18.09.2017 18:26:03
ISBN/EAN: 3844399291

ISBN - Autres types d'écriture:
3-8443-9929-1, 978-3-8443-9929-5


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