ISBN: 9781617379390
Hearing is a sensory modality critical to both language and cognitive development. In its absence, and without sensory input through another modality, such as the manual/visual modality o… Plus…
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Methods and Protocols, Buch, Softcover, Softcover reprint of hardcover 1st ed. 2009, [PU: Humana Press Inc.], Humana Press Inc., 2011
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ISBN: 9781617379390
Hearing is a sensory modality critical to both language and cognitive development. In its absence, and without sensory input through another modality, such as the manual/visual modality o… Plus…
Sokolowski, Bernd (Herausgeber):
Auditory and Vestibular Research Methods and Protocols - Livres de poche2011, ISBN: 1617379395
Edition reliée
Softcover reprint of hardcover 1st ed. 2009 Kartoniert / Broschiert Physiologie, Pathologie, Neurologie und klinische Neurophysiologie, Genetik (nicht-medizinisch), Cells; gene expressi… Plus…
2011
ISBN: 1617379395
Edition reliée
Softcover reprint of hardcover 1st ed. 2009 Kartoniert / Broschiert Physiologie, Pathologie, Neurologie und klinische Neurophysiologie, Genetik (nicht-medizinisch), molecularbiology; ph… Plus…
2011, ISBN: 9781617379390
Methods and Protocols, Buch, Softcover, Softcover reprint of hardcover 1st ed. 2009, [PU: Humana Press Inc.], Humana Press Inc., 2011
ISBN: 9781617379390
Livre
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Informations détaillées sur le livre - Auditory and Vestibular Research
EAN (ISBN-13): 9781617379390
ISBN (ISBN-10): 1617379395
Version reliée
Livre de poche
Date de parution: 2011
Editeur: Humana Press Inc.
440 Pages
Langue: eng/Englisch
Livre dans la base de données depuis 2011-09-04T14:46:15+02:00 (Paris)
Page de détail modifiée en dernier sur 2024-03-26T20:36:20+01:00 (Paris)
ISBN/EAN: 1617379395
ISBN - Autres types d'écriture:
1-61737-939-5, 978-1-61737-939-0
Autres types d'écriture et termes associés:
Auteur du livre: sokolowski, sokol, sokolow, bernd
Données de l'éditeur
Auteur: Bernd Sokolowski
Titre: Methods in Molecular Biology; Auditory and Vestibular Research - Methods and Protocols
Editeur: Humana; Humana Press
428 Pages
Date de parution: 2011-01-20
Totowa; NJ; US
Imprimé / Fabriqué en
Langue: Anglais
155,14 € (DE)
159,49 € (AT)
171,50 CHF (CH)
POD
XII, 428 p. 61 illus., 13 illus. in color.
BC; Hardcover, Softcover / Biologie; Physiologie; Verstehen; cells; gene expression; gene transfer; genes; hybridization; microscopy; molecular biology; physiology; regulation; tissue; Human Physiology; Neurology; Biomedical Research; Pathology; Molecular Genetics; Neurologie und klinische Neurophysiologie; Medizinische Forschung; Pathologie; Genetik (nicht-medizinisch); BB
Nucleic Acid Protocols.- RNA Isolation from Xenopus Inner Ear Sensory Endorgans for Transcriptional Profiling and Molecular Cloning.- Synthesis of Biotin-Labeled RNA for Gene Expression Measurements Using Oligonucleotide Arrays.- In situ Hybridization Approach to Study mRNA Expression and Distribution in Cochlear Frozen Sections.- Lineage Analysis of Inner Ear Cells Using Genomic Tags for Clonal Identification.- Genetic Fate-Mapping Approaches: New Means to Explore the Embryonic Origins of the Cochlear Nucleus.- The Practical Use of Cre and loxP Technologies in Mouse Auditory Research.- Helios® Gene Gun–Mediated Transfection of the Inner Ear Sensory Epithelium.- Electroporation-Mediated Gene Transfer to the Developing Mouse Inner Ear.- Isolation of Sphere-Forming Stem Cells from the Mouse Inner Ear.- Molecular Biology of Vestibular Schwannomas.- Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis Media Causing Pathogens.- Fluorescence “In Situ” Hybridization for the Detection of Biofilm in the Middle Ear and Upper Respiratory Tract Mucosa.- Positional Cloning of Deafness Genes.- Amino Acid Protocols.- Twist-Off Purification of Hair Bundles.- Yeast Two-Hybrid Screening to Test for Protein–Protein Interactions in the Auditory System.- The Use of 2-D Gels to Identify Novel Protein–Protein Interactions in the Cochlea.- Identification of Functionally Important Residues/Domains in Membrane Proteins Using an Evolutionary Approach Coupled with Systematic Mutational Analysis.- In Vivo Verification of Protein Interactions in the Inner Ear by Coimmunoprecipitation.- Identification of Transcription Factor–DNA Interactions Using Chromatin Immunoprecipitation Assays.- Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Proteins inInner-Ear Sensory Epithelia.- Multiplexed Isobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches to Auditory Research.- Imaging Protocols.- Fluorescence Microscopy Methods in the Study of Protein Structure and Function.- Ion Imaging in the Cochlear Hair Cells.- Atomic Force Microscopy in Studies of the Cochlea.Lists all the reagents, step-by-step techniques, and limitations for reproducible results in DNA/RNA tracing, protein identification, and microscopic imaging Clearly describes the protocols necessary for in vivo and in vitro gene delivery techniques Provides the tools to identify and isolate cochlear stem cells for the treatment of inner ear pathologies Includes lineage and fate mapping techniques for peripheral and central studies of auditory/vestibular development Describes techniques for identification and verification of protein-protein interactions Provides important tools for the basic and clinical researcher to identify genes and microbes underlying diseases of the ear including the cloning of deafness genes Includes supplementary material: sn.pub/extras
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