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Protein NMR Techniques - A. Kristina Downing (Editor)
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A. Kristina Downing (Editor):
Protein NMR Techniques - nouveau livre

ISBN: 9781617374494

Carrying on in the tradition of its much praised earlier edition, A. Kristina Downing has selected new prools describing the many recent advances in NMR methodology that are revolutionizing the field. Described in step-by-step detail by established hands-on experts, these readily reproducible techniques include methods for high-level recombinant protein expression using sophisticated isotopic labeling strategies, TROSY methods for the study of structure and dynamics, and methods for the acquisition and interpretation of residual dipolar coupling data. There are also applications of dynamics measurements on multiple timescales, new developments in NMR data analysis, structure calculation prools, and a comprehensive chapter on solid-state methods for the study of membrane proteins. The prools follow the successful Methods in Molecular Biology™ series format, each offering detailed laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.State-of-the-art and highly practical, Protein NMR Techniques, 2nd Ed., makes accessible to all NMR laboratories proven techniques that are powerfully transforming the speed, accuracy, and size limit of our ability to characterize biological macromolecules. Textbooks New Books ~~ Science~~ Life Sciences ~~ Biochemistry Protein-NMR-Techniques~~A-Kristina-Downing Springer-Verlag New York, LLC This collection of readily reproducible methods using the latest advances in NMR technology includes methods for high-level recombinant protein expression using sophisticated isotopic labeling strategies, TROSY methods for the study of structure and dynamics, and methods for the acquisition and interpretation of residual dipolar coupling data. There are also applications of dynamics measurements on multiple timescales, new developments in NMR data analysis, structure calculation protocols, and solid-state methods. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering detailed laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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Protein Nmr Techniques
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Protein Nmr Techniques - nouveau livre

ISBN: 9781617374494

ID: 16549521

When I was asked to edit the second edition of Protein NMR Techniques, my first thought was that the time was ripe for a new edition. The past several years have seen a surge in the development of novel methods that are truly revolutionizing our ability to characterize biological macromolecules in terms of speed, accuracy, and size limitations. I was particularly excited at the prospect of. When I was asked to edit the second edition of Protein NMR Techniques, my first thought was that the time was ripe for a new edition. The past several years have seen a surge in the development of novel methods that are truly revolutionizing our ability to characterize biological macromolecules in terms of speed, accuracy, and size limitations. I was particularly excited at the prospect of making these techniques accessible to all NMR labs and for the opportunity to ask the experts to divulge their hints and tips and to write, practically, about the methods. I commissioned 19 chapters with wide scope for Protein NMR Techniques, and the volume has been organized with numerous themes in mind. Chapters 1 and 2 deal with recombinant protein expression using two organisms, E. coli and P. pastoris, that can produce high yields of isotopically labeled protein at a reasonable cost. Staying with the idea of isotopic labeling, Chapter 3 describes methods for perdeuteration and site-specific protonation and is the first of several chapters in the book that is relevant to studies of higher molecular weight systems. A different, but equally powerful, method that uses molecular biology to "edit" the spectrum of a large molecule using segmental labeling is presented in Chapter 4. Having successfully produced a high molecular weight target for study, the next logical step is data acquisition. Hence, the final chapter on this theme, Chapter 5, describes TROSY methods for stru- ural studies. Books, Science and Geography~~Biology, Life Sciences~~Biochemistry, Protein Nmr Techniques~~Book~~9781617374494, , , , , , , , , ,

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Protein NMR Techniques
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Protein NMR Techniques - Livres de poche

2010, ISBN: 1617374490, Lieferbar binnen 4-6 Wochen Frais d'envoiVersandkostenfrei innerhalb der BRD

ID: 9781617374494

Internationaler Buchtitel. In englischer Sprache. Verlag: SPRINGER VERLAG GMBH, 496 Seiten, L=229mm, B=154mm, H=35mm, Gew.=732gr, Softcover of Or. [GR: 26760 - TB/Biochemie/Biophysik], [SW: - Science], Kartoniert/Broschiert, Klappentext: This collection of readily reproducible methods using the latest advances in NMR technology includes methods for high-level recombinant protein expression using sophisticated isotopic labeling strategies, TROSY methods for the study of structure and dynamics, and methods for the acquisition and interpretation of residual dipolar coupling data. There are also applications of dynamics measurements on multiple timescales, new developments in NMR data analysis, structure calculation protocols, and solid-state methods. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering detailed laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. [Ausgabe: 0002] This collection of readily reproducible methods using the latest advances in NMR technology includes methods for high-level recombinant protein expression using sophisticated isotopic labeling strategies, TROSY methods for the study of structure and dynamics, and methods for the acquisition and interpretation of residual dipolar coupling data. There are also applications of dynamics measurements on multiple timescales, new developments in NMR data analysis, structure calculation protocols, and solid-state methods. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering detailed laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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Protein NMR Techniques - A. Kristina Downing
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A. Kristina Downing:
Protein NMR Techniques - Livres de poche

2010, ISBN: 9781617374494

ID: 16168417

2nd ed. Softcover of orig. ed. 2004, Softcover, Buch, [PU: Humana Press Inc.]

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Protein NMR Techniques 2nd Edition - NA
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NA:
Protein NMR Techniques 2nd Edition - nouveau livre

ISBN: 9781617374494

ID: 842355167

NA, Humana Press

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Détails sur le livre
Protein NMR Techniques

This collection of readily reproducible methods using the latest advances in NMR technology includes methods for high-level recombinant protein expression using sophisticated isotopic labeling strategies, TROSY methods for the study of structure and dynamics, and methods for the acquisition and interpretation of residual dipolar coupling data. There are also applications of dynamics measurements on multiple timescales, new developments in NMR data analysis, structure calculation protocols, and solid-state methods. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering detailed laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Informations détaillées sur le livre - Protein NMR Techniques


EAN (ISBN-13): 9781617374494
ISBN (ISBN-10): 1617374490
Livre de poche
Date de parution: 2010
Editeur: SPRINGER VERLAG GMBH
496 Pages
Poids: 0,732 kg
Langue: eng/Englisch

Livre dans la base de données depuis 06.10.2011 11:12:21
Livre trouvé récemment le 07.05.2017 18:23:59
ISBN/EAN: 1617374490

ISBN - Autres types d'écriture:
1-61737-449-0, 978-1-61737-449-4


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