ISBN: 9781493953295
[ED: Softcover], [PU: Springer / Springer, Berlin], Directed Evolution Library Creation: Methods and Protocols, Second Edition presents user-friendly protocols for both proven strategies … Plus…
| booklooker.de |
2016, ISBN: 149395329X
[EAN: 9781493953295], Neubuch, [PU: Springer New York], DELETIONMUTATIONS; DIRECTEDEVOLUTION; GENEVARIANTLIBRARIES; INSERTIONMUTATIONS; GENETICENGINEERING; MUTAGENESIS, Druck auf Anfrage … Plus…
| AbeBooks.de AHA-BUCH GmbH, Einbeck, Germany [51283250] [Rating: 5 (von 5)] NEW BOOK. Frais d'envoiVersandkostenfrei. (EUR 0.00) Details... |
2016, ISBN: 149395329X
[EAN: 9781493953295], Neubuch, [PU: Springer-Verlag New York Inc., United States], Language: English. Brand new Book. Directed Evolution Library Creation: Methods and Protocols, Second Ed… Plus…
| AbeBooks.de The Book Depository, London, United Kingdom [54837791] [Rating: 5 (von 5)] NEW BOOK. Frais d'envoi EUR 0.57 Details... |
2016, ISBN: 149395329X
[EAN: 9781493953295], Neubuch, [PU: Springer-Verlag New York Inc., United States], Language: English. Brand new Book. Directed Evolution Library Creation: Methods and Protocols, Second Ed… Plus…
| AbeBooks.de Book Depository hard to find, London, United Kingdom [63688905] [Rating: 5 (von 5)] NEW BOOK. Frais d'envoi EUR 1.00 Details... |
2016, ISBN: 9781493953295
Methods and Protocols, Buch, Softcover, Softcover reprint of the original 2nd ed. 2014, [PU: Springer-Verlag New York Inc.], Springer-Verlag New York Inc., 2016
| lehmanns.de Frais d'envoiDoes not ship to your country., Livraison non-comprise Details... |
ISBN: 9781493953295
[ED: Softcover], [PU: Springer / Springer, Berlin], Directed Evolution Library Creation: Methods and Protocols, Second Edition presents user-friendly protocols for both proven strategies … Plus…
Elizabeth M. J. Gillam:
Directed Evolution Library Creation : Methods and Protocols - Livres de poche2016, ISBN: 149395329X
[EAN: 9781493953295], Neubuch, [PU: Springer New York], DELETIONMUTATIONS; DIRECTEDEVOLUTION; GENEVARIANTLIBRARIES; INSERTIONMUTATIONS; GENETICENGINEERING; MUTAGENESIS, Druck auf Anfrage … Plus…
2016
ISBN: 149395329X
[EAN: 9781493953295], Neubuch, [PU: Springer-Verlag New York Inc., United States], Language: English. Brand new Book. Directed Evolution Library Creation: Methods and Protocols, Second Ed… Plus…
2016, ISBN: 149395329X
[EAN: 9781493953295], Neubuch, [PU: Springer-Verlag New York Inc., United States], Language: English. Brand new Book. Directed Evolution Library Creation: Methods and Protocols, Second Ed… Plus…
2016, ISBN: 9781493953295
Methods and Protocols, Buch, Softcover, Softcover reprint of the original 2nd ed. 2014, [PU: Springer-Verlag New York Inc.], Springer-Verlag New York Inc., 2016
Données bibliographiques du meilleur livre correspondant
| Auteur: | |
| Titre: | |
| ISBN: |
Directed Evolution Library Creation: Methods and Protocols, Second Edition presents user-friendly protocols for both proven strategies and cutting-edge approaches for the creation of mutant gene libraries for directed evolution. As well as experimental methods, information on current computational approaches is provided in a user-friendly format that will allow researchers to make informed choices without needing to comprehend the full technical details of each algorithm. Directed evolution has become a fundamental approach for engineering proteins to enhance activity and explore structure-function relationships, and has supported the rapid development of the field of synthetic biology over the last decade. Divided into three convenient sections, topics include point mutagenesis strategies, recombinatorial methods wherein genetic diversity is sourced from multiple parental genes that are combined via either homology-dependent or -independent techniques and a variety of computational methods to guide the design and analysis of mutant libraries. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Directed Evolution Library Creation: Methods and Protocols, Second Edition will serve as a reliable manual for both novice and experienced protein engineers and synthetic biologists and will enable further technical innovation and the exploitation of directed evolution for a deeper understanding of protein design and function.
Informations détaillées sur le livre - Directed Evolution Library Creation
EAN (ISBN-13): 9781493953295
ISBN (ISBN-10): 149395329X
Version reliée
Livre de poche
Date de parution: 2016
Editeur: Springer-Verlag New York Inc.
Livre dans la base de données depuis 2016-10-04T21:15:00+02:00 (Paris)
Page de détail modifiée en dernier sur 2023-06-19T04:29:00+02:00 (Paris)
ISBN/EAN: 9781493953295
ISBN - Autres types d'écriture:
1-4939-5329-X, 978-1-4939-5329-5
Autres types d'écriture et termes associés:
Auteur du livre: elizabeth david, ackerl, gillam, copp
Titre du livre: creatio, evolution, dmc creation, library
Données de l'éditeur
Auteur: Elizabeth M.J. Gillam; Janine N. Copp; David Ackerley
Titre: Methods in Molecular Biology; Directed Evolution Library Creation - Methods and Protocols
Editeur: Springer; Springer US
369 Pages
Date de parution: 2016-09-17
New York; NY; US
Imprimé / Fabriqué en
Langue: Anglais
117,69 € (DE)
120,99 € (AT)
130,00 CHF (CH)
POD
XI, 369 p. 63 illus., 19 illus. in color.
BC; Hardcover, Softcover / Medizin/Klinische Fächer; Genetik, Medizin; Verstehen; deletion mutations; directed evolution; gene variant libraries; insertion mutations; mutagenesis; genetic engineering; Medical Genetics; Evolutionary Biology; Chemical Bioengineering; Evolution; Biotechnologie; BB
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
will serve as a reliable manual for both novice and experienced protein engineers and synthetic biologists and will enable further technical innovation and the exploitation of directed evolution for a deeper understanding of protein design and function.
Directed Evolution Library Creation: Methods and Protocols, Second Edition Methods in Molecular Biology Directed Evolution Library Creation: Methods and Protocols, Second EditionError-Prone PCR and Effective Generation of Gene Variant Libraries for Directed Evolution.- Error-Prone Rolling Circle Amplification Greatly Simplifies Random Mutagenesis.- Random Mutagenesis by Error-Prone Pol Plasmid Replication in Escherichia coli.- The Sequence Saturation Mutagenesis (SeSaM) Method.- Generation of Effective Libraries by Neutral Drift.- Site Saturation Mutagenesis.- Iterative Saturation Mutagenesis (ISM): A Powerful Approach to Engineer Proteins by Systematically Simulating Darwinian Evolution.- Generating Targeted Libraries by the Combinatorial Incorporation of Synthetic Oligonucleotides During Gene Shuffling (ISOR).- OmniChange: Simultaneous Site-Saturation of up to Five Codons.- Random Insertional–Deletional Strand Exchange Mutagenesis (RAISE): A Simple Method for Generating Random Insertion and Deletion Mutations.- Transposon-Based Approaches for Generating Novel Molecular Diversity during Directed Evolution.- Restriction Enzyme-Mediated DNA Family Shuffling.- Assembly of Designed Oligonucleotides (ADO): A Useful Tool in Synthetic Biology for Creating High Quality Combinatorial DNA Libraries.- One-Pot, Simple Methodology for Cassette Randomization and Recombination for Focused Directed Evolution (OSCARR).- USER Friendly DNA Recombination (USERec): Gene Library Construction Requiring Minimal Sequence Homology.- ITCHY: Incremental Truncation for the Creation of Hybrid Enzymes.- Generating Random Circular Permutation Libraries.- Probabilistic Methods in Directed Evolution: Library Size, Mutation Rate and Diversity.- The Mutagenesis Assistant Program.- Computational Tools for Designing Smart Libraries.- Computational Tools for Directed Evolution: A Comparison of Prospective and Retrospective Strategies.- Designing Libraries of Chimeric Proteins Using SCHEMA Recombination and RASPP.- Non-Contiguous SCHEMA Protein Recombination.- Engineering Proteins by Reconstructing Evolutionary Adaptive Paths.
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
will serve as a reliable manual for both novice and experienced protein engineers and synthetic biologists and will enable further technical innovation and the exploitation of directed evolution for a deeper understanding of protein design and function.
Directed Evolution Library Creation: Methods and Protocols, Second Edition Methods in Molecular Biology Directed Evolution Library Creation: Methods and Protocols, Second EditionIncludes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts
Autres livres qui pourraient ressembler au livre recherché:
Dernier livre similaire:
9781493910526 Directed Evolution Library Creation (Herausgegeben:Gillam, Elizabeth M.J.; Copp, Janine N.; Ackerley, David)
- 9781493910526 Directed Evolution Library Creation (Herausgegeben:Gillam, Elizabeth M.J.; Copp, Janine N.; Ackerley, David)
- 9781617374715 Directed Evolution Library Creation: Methods and Protocols (Methods in Molecular Biology) (Methods in Molecular Biology, 231, Band 231) (Frances H. Arnold)
- 9781588292858 Directed Evolution Library Creation (Frances H. Arnold; George Georgiou)
< pour archiver...